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A new vaccination regimen using adenovirus-vectored vaccine confers effective protection against African swine fever virus in swine

Emerg Microbes Infect .. 2023-12;

Wenming Liu , Hengchun Li , Bo Liu , Tianxing Lv , Chenchen Yang , Si Chen , Liqiang Feng , Liangxue Lai , Ziyuan Duan , Xinwen Chen , Pingchao Li , Suhua Guan , Ling Chen

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Peptide Synthesis	5 × 105 cells per well were seeded and stimulated with the corresponding peptide pool (p30, p54, CD2v, or p72) (Table S1) at 2 μg ml−1 per peptide (Genescript, China) for 24 h.	Get A Quote

摘要

African swine fever (ASF) is an acute and highly contagious lethal infectious disease in swine that severely threatens the global pig industry. At present, a safe and efficacious vaccine is urgently required to prevent and control the disease. In this study, we evaluated the safety and immunogenicity of replication-incompetent type-2 adenoviruses carrying African swine fever virus (ASFV) antigens, namely CP204L (p30), E183L (p54), EP402R (CD2v), B646L (p72), and B602L (p72 chaperone). A vaccine cocktail delivered by simultaneous intramuscular (IM) and intranasal (IN) administration robustly elicited both systemic and mucosal immune responses against AFSV in mice and swine and provided highly effective protectio... More